BACTERIOPHAGE LAMBDA PHAGE HAVE THE ABILITY TO SURVIVE EITHER AS A LYSOGEN W/IN A BACTERIAL HOST OR BY LYTIC GROWTH.

BACTERIOPHAGE LAMBDA PHAGE HAVE THE ABILITY TO SURVIVE EITHER AS A LYSOGEN W/IN A BACTERIAL HOST OR BY LYTIC GROWTH.

LYTIC GROWTH INVOLVES REPLICATION OF THE VIRAL DNA MANYFOLD, ASSEMBLY OF BACTERIOPHAGE PARTICLES, AND CELL LYSIS TO RELEASE THE NEW INFECTIOUS BACTERIOPHAGE PARTICLES. THE BACTERIOPHAGE PARTICLES CAN EACH PACKAGE ONE ENTIRE VIRAL GENOME OF ~50KB BUT NOT MUCH MORE DNA THAN THAT. USE YOUR KNOWLEDGE OF THE REGULATION OF THE LAMBDA GENE EXPRESSION TO DESIGN A LAMBDA CLONING VECTOR. THE CLONING VECTOR SHOULD BE REPLICATED MANYFOLD, AND ASSEMBLE INTO INFECTIOUS VIRAL PARTICLES THAT ARE RELEASED BY LYSIS OF THE HOST BACTERIAL CELL. HINT: THINK ABOUT WHAT GENES ARE NECESSARY FOR LYSOGENIC GROWTH AND DISPENSABLE FOR LYTIC GROWTH. THESE ARE THE GENES THAT YOU CAN REPLACE WITH YOUR DNA OF INTEREST.

Bacteriophage Lambda phage have the ability to survive either as a lysogen w/in a bacterial host or by lytic growth. Lytic growth involves replication of the viral DNA manyfold, assembly of bacteriophage particles, and cell lysis to release the new infectious bacteriophage particles. The bacteriophage particles can each package one entire viral genome of ~50Kb but not much more DNA than that. Use your knowledge of the regulation of the lambda gene expression to design a lambda cloning vector. The cloning vector should be replicated manyfold, and assemble into infectious viral particles that are released by lysis of the host bacterial cell. Hint: think about what genes are necessary for lysogenic growth and dispensable for lytic growth. These are the genes that you can replace with your DNA of interest.

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